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RhC7/pSVL

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編號:B193841

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產品名稱	RhC7/pSVL
商品貨號	B193841
Designations	RhC7/pSVL
Species	Homo sapiens, human
Applications	When cotransfected into CHO cells with pSV2neo, the construct expresses the receptor.
Vector	Construct size (kb): 6.400000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSVL Intact vector size: 4.889 Type of vector: plasmid Vector end: XhoI Vector end: XbaI Cloning sites: XhoI XbaI SmaI XmaI SacI BamHI Polylinker sites: XhoI XbaI SmaI XmaI SacI BamHI Construction: pBR322, SV40 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: SV40 late replicon: pMB1, SV40 terminator: SV40 late poly(A) Cross references:
Insert	DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ADORA1 Genomic copy number: unique Gene name: adenosine A1 receptor Contains complete coding sequence?: Y Chromosome: 1; Localization: 1 q32.1 Tissue: hippocampus Type of DNA: cDNA Insert 5' end: PstI Insert 3' end: XbaI Insert size (kb): 1.5 Cross references: Insert lengths(kb): 1.5 Tissue: hippocampus Gene product: adenosine A1 receptor [ADORA1]
Insert Size (kb)	1.5
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments	Restriction digests of the clone give the following sizes (kb): PstI/XbaI--3.3, 2.3, 1.9; BamHI--6.8, 0.9; EcoRI--5.4, 2.3; HindIII--5.8, 1.35, 0.6; XhoI--7.8. When cotransfected into CHO cells with pSV2neo, the construct expresses the receptor. The construct includes an XhoI/PstI fragment derived from a pBluescript SK+ multiple cloning sequence. The PstI site is at the 5' end of the cDNA. The PstI/XbaI fragment includes 350 bp of 5' untranslated and 220 bp of 3' untranslated sequence.
References	Libert F, et al. Cloning and functional characterization of a human A1 adenosine receptor. Biochem. Biophys. Res. Commun. 187: 919-926, 1992. PubMed: 1530647 Gilbert Vassart, personal communication

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