| Vector |
Construct size (kb): 0.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873 |
| Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 2.587, 3.039, 2.677, 1.558, 8.117, 1.363, 4.928, 2.186, 1.342, 4.237. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 1.4, 1.6, 2.3, 2.8, 3.3, 4.4, 5.3, 8.9. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70449, 70515. |
