| 產(chǎn)品名稱 | Quantitative Synthetic Dengue virus type 4 RNA |
|---|---|
| 商品貨號(hào) | B193530 |
| Agent | Quantitative Synthetic Dengue virus type 4 RNA |
| Applications | ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day to day test variation, and lot to lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen Specification range: 1 x 105 - 1 x 106 copies/μL 100 µL per vial with Biomatrica RNAstable |
| Storage Conditions | -70°C or colder |
| Intended Use | The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent. |
| Comments | Manufactured under ISO 13485 guidance Preparation includes fragments from the capsid, membrane, and envelope regions The following primers and probe can be used with this nucleic acid preparation Ref   Centers for Disease Control and Prevention (CDC). CDC DENV-1-4 Real-Time RT-PCR Assay for Detection and Serotype Identification of Dengue Virus. Instructions for Use Package Insert.:Forward primer: TTGTCCTAATGATGCTRGTCG Reverse primer: TCCACCYGAGACTCCTTCCA Probe: TYCCTACYCCTACGCATCGCATTCCG |
| Special Collection | RNA |
| References | Waggoner JJ, et al. Development of an internally controlled real-time reverse transcriptase PCR assay for pan-dengue virus detection and comparison of four molecular dengue virus detection assays. J. Clin. Microbiol. 51(7): 2172-2181, 2013. PubMed: 23637298 Waggoner JJ, et al. Single-reaction, multiplex, real-time rt-PCR for the detection, quantitation, and serotyping of dengue viruses. PLoS Negl. Trop. Dis. 7(4): e2116, 2013. PubMed: 23638191 Centers for Disease Control and Prevention (CDC). CDC DENV-1-4 Real-Time RT-PCR Assay for Detection and Serotype Identification of Dengue Virus. Instructions for Use Package Insert. |
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