Clostridium perfringens (Veillon and Zuber) Hauduroy et al.
商品貨號
B193519
Strain Designations
Strain 34. Type b [A.J. Wilsdon type B, strain 34, L.S. McClung 1955, NCIB 10691]
Isolation
Intestinal contents of lamb
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Preceptrol®
no
Genotype
The presence of cpa, cpb, etx, cpb2, and pfoA genes was confirmed by PCR.
Type Strain
no
Comments
Type B
The presence of cpa, cpb, etx, cpb2, and pfoA genes was confirmed by PCR (assay 2, 3).
Produces hemolysins, lethal toxins, phospholipase C, and lecithinase C.
In 24 to 48 hours, growth is evident by turbidity and gas formation in the broth and by colonies on the anaerobic agar surfaces. No growth will occur on agar plate incubated aerobically.
Medium
ATCC® Medium 2107: Modified Reinforced Clostridial
ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic
Name of Depositor
IC Hall
References
Mollby R, Holme T. Production of phospholipase C (alpha-toxin), haemolysins and lethal toxins by Clostridium perfringens types A to D. J. Gen. Microbiol. 96: 137-144, 1976. PubMed: 10344
Meer RR, Songer JG. Multiplex polymerase chain reaction assay for genotyping Clostridium perfringens. Am. J. Vet. Res. 58(7): 702-705, 1997. PubMed: 9215442
Garmory HS, et al. Occurrence of Clostridium perfringens beta2-toxin amongst animals, determined using genotyping and subtyping PCR assays. Epidemiol. Infect. 124(1): 61-67, 2000. PubMed: 10722131
