| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PCK1
Gene name: phosphoenolpyruvate carboxykinase 1
Contains complete coding sequence?: Y
Chromosome: 20; Localization: 20 q13.2-q13.31
Tissue: kidney
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.7
Cross references: DNA Seq. Acc.: L05144 Insert lengths(kb): 2.700000047683716 Tissue: kidney Gene product: phosphoenolpyruvate carboxykinase 1, soluble(phosphoenolpyruvate carboxykinase (GTP)) [PCK1] |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--16.0, 9.0, 6.8 (doublet), 5.6, 1.5; EcoRI--32.0, 11.0, 2.0, 0.8; HindIII--23.0, 9.3 (doublet), 4.5; NotI--34.0, 12.0; PstI--11.0, 5.6 (doublet), 2.8 (doublet), 2.5 (doublet), 2.1, 1.8, 1.5, 1.3 (doublet, 1.2, 1.0 (doublet), 0.85; PvuII--11.0 (doublet), 4.5 (doublet), 4.1, 3.8, 1.8, 1.4; SacI--33.0, 13.0. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.15, 1.52; PvuII--0.13, 1.47; NotI--0.97; SacI--1.3; EcoRI--1.87. Contains the complete coding sequence (nt 122-1990). The 3' untranslated region contains a simple tandem repeat polymorphism that is highly polymorphic. |
| References |
Stoffel M, et al. cDNA sequence and localization of polymorphic human cytosolic phosphoenolpyruvate carboxykinase gene (PCK1) to chromosome 20, band q13.31: PCK1 is not tightly linked to maturity-onset diabetes of the young. Hum. Mol. Genet. 1: 1-4, 1993. PubMed: 8490617
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