| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--23.0, 8.3, 7.0, 2.3; EcoRI--20.0, 8.5 (doublet), 2.5, 2.3, 1.4, 0.98; HindIII--7.0, 6.0, 5.4, 4.2, 3.9, 3.3, 3.2, 2.5, 2.0, 1.8, 1.7, 1.6, 1.0, 0.98; BglII--15.0, 7.2, 6.6, 6.0, 5.4, 3.2, 2.2, 1.6, 1.5, 1.4; SalI--26.0, 23.1, 2.7. Codominant segregation demonstrated in 60 families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: PstI, MspI. |
