| Comments |
The insert contains HindIII, SmaI (3), PvuII (3), HIndII, and PvuI sites. Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 3.0; HindIII--7.4; PvuII--2.7, 2.3, 1.5, 0.9. The insert contains a 0.8 kb PvuII fragment which was subcloned into pBR322 to form pPvuIIE (ATCC 41006). Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
| References |
DeLorbe WJ, et al. Molecular cloning and characterization of avian sarcoma virus circular DNA molecules. J. Virol. 36: 50-61, 1980. PubMed: 6255214
Barbara Weiffenbach, personal communication
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