| 產品名稱 |
pCI-Rat-NFL |
| 商品貨號 |
B192889 |
| Designations |
pCI-Rat-NFL |
| GenBank Number |
AF031880
|
| Species |
Rattus norvegicus, rat |
| Depositors |
RK Liem |
| Insert |
DNA: cDNA Insert lengths(kb): 2.0 Tissue: brain, 21-day-old Gene product: neurofilament, light polypeptide (68kD)( neurofilament light protein, neurofilament protein, light polypeptide) [Nefl] |
| Insert Size (kb) |
2.0 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglII--6.0; EcoRI--3.4, 1.75;EcoRI/NotI--3.5, 1.8. Insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.05; BglII--0.80; KpnI--1.17; PvuI--0.37; PvuII--0.35, 1.07; SacI--1.32; SmaI--0.04, 0.14; XbaI--1.06; XhoI--1.38; XhoII--0.80. |
| References |
Chin SS, Liem RK. Expression of rat neurofilament proteins NF-L and NF-M in transfected non-neuronal cells. Eur. J. Cell Biol. 50: 475-490, 1989. PubMed: 2516804
Leung CL, et al. The intermediate filament protein peripherin is the specific interaction partner of mouse BPAG1-n (Dystonin) in neurons. J. Cell Biol. 144: 435-446, 1999. PubMed: 9971739
|
