| Vector |
Construct size (kb): 5.099999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKAR1B
Gene name: protein kinase, cAMP-dependent, regulatory, type I, beta
Contains complete coding sequence?: U
Localization: 7 pter-p22
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: M85708; EST: EST02227; Primer: M13 Forward;
THC id: THC87394 Insert lengths(kb): 2.099999904632568 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: protein kinase, cAMP-dependent, regulatory, type I, beta [PRKAR1B] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.1; PstI--3.4, 1.4, 0.3; HindIII--3.6, 1.5. EST02227 matches GenBank accession HUMRIB with 94.90% identity over the sequence range: 1-255. |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
J C Venter, personal communication
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