Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; HindIII--2.7; NcoI--2.7.
Expression vector. Permits high copy number and production of proteins initiated at the ATG of beta-galactosidase.
The order of the major features in the plasmid is: pMB1 ori - EcoRI - trc promoter - lacZ'/NcoI - PstI - HindIII - ampR.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Beernink PT, Tolan DR. Construction of a high-copy "ATG vector" for expression in Escherichia coli. Protein Expr. Purif. 3: 332-336, 1992. PubMed: 1422227
Shipped
frozen
Shipping Information
Frozen glycerol stock of ?E. coli containing the plasmid
