| 產品名稱 |
145-12 |
| 商品貨號 |
B191902 |
| Designations |
145-12 |
| Species |
Homo sapiens, human |
| Depositors |
LM Kunkel |
| Applications |
The RFLP-detecting clone is generated by double digestion of the insert with HindIII and EcoRI. The unique sequence fragments to be used for hybridization are the 500 bp doublet. |
| Vector |
Construct size (kb): 12.39999961853027 |
| Insert |
DNA: genomic Insert lengths(kb): 8.0 Gene product: DNA Segment, single copy [DXS141] Alleles: A1, A2, B1, B2 |
| Insert Size (kb) |
8.0 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): SalI--12.4; PstI--6.2, 4.8, 1.4; BamHI--12.4; HindIII--8.0, 4.4; EcoRI--6.0, 3.9, 2.5. Part of a phage walk taken with the original pERT145 clone. This is one of the most proximal pERT clones absent from the BB deletion. The RFLP-detecting clone is generated by double digestion of the insert with HindIII and EcoRI. The unique sequence fragments to be used for hybridization are the 500 bp doublet. The remaining fragments are slightly repeated. |
| References |
Bertelson CJ, et al. Localisation of Xp21 meiotic exchange points in Duchenne muscular dystrophy families. J. Med. Genet. 23: 531-537, 1986. PubMed: 2879924
Kunkel LM, et al. Specific cloning of DNA fragments absent from the DNA of a male patient with an X chromosome deletion. Proc. Natl. Acad. Sci. USA 82: 4778-4782, 1985. PubMed: 2991893
Louis M Kunkel, personal communication
|
