| Vector Information |
Size (kb): 7.8000001907348630 Vector: YIp28 (plasmid) Construction: pBR322, Saccharomyces cerevisiae Marker(s):LEU2,URA3,ampR Construct size (kb): 7.800000190734863 Features: marker(s): ampR, LEU2, URA3
replicon: pMB1 |
| Comments |
A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector. Orientations are such that transcription from URA3 is clockwise and from LEU2 is counterclockwise. A 1.1 kb HindIII fragment containing URA3 and a 2.2 kb SalI/XhoI fragment containing LEU2 were cloned into the HindIII and SalI sites, respectively, of pBR322. Unique restriction sites are in the 3' untranslated regions of URA3(SmaI) and LEU2(SalI). |
| References |
Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030
Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863
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