| Comments |
Restriction digests of the clone give the following sizes (kb): KpnI--8.0; XhoI--5.6,2.4; PvuII--2.3, 2.0, 2.0, 0.74, 0.6, 0.4; EcoRI--3.6, 2.0, 1.6, 0.8; KpnI/NotI--4.1, 3.9. The insert contains the following restriction sites (approximate from the 5' end): BamHI--1.97, 2.48; EcoRI--0.72, 2.30; EcoRV--3.87, 4.01; HindIII--2.67; NcoI--0.07, 2.55; NdeI--0.96, 1.75; PvuII--0.39, 2.42; SacI--3.83; XbaI--2.06; XhoI--2.38. |
