| 產品名稱 | RWPE2-W99 |
|---|---|
| 商品貨號 | B190766 |
| Organism | Homo sapiens, human |
| Tissue | prostate |
| Cell Type | epithelial |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 2 cells containing human HPV-18 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | 54 years |
| Gender | male |
| Ethnicity | Caucasian, White |
| Applications | The RWPE2-W99 cell line was derived, in 1999, from the RWPE-2 (ATCC CRL-11610) cell line by cloning in soft agar to select cells that show high expression of Ki-ras. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | The depositor reports that at passage 44, a majority of the cells had a near diploid chromosome number of 48; X, -Y. |
| Images |  |
| Derivation | The RWPE2-W99 cell line was derived, in 1999, from the RWPE-2 (ATCC CRL-11610) cell line by cloning in soft agar to select cells that show high expression of Ki-ras. RWPE2-W99 cells show strong expression of Ki-ras and have characteristics similar to those of RWPE-2 cells.
RWPE-2 cells were derived from RWPE-1 cells (ATCC CRL-11609) by transformation with Ki-ras using the Kirsten murine sarcoma virus (Ki-MuSV) to establish the RWPE-2 cells (PubMed: 9214605). Epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome to establish the RWPE-1 cell line (ATCC CRL-11609). A family of tumorigenic cell lines that mimics multiple steps in prostate cancer progression was also derived from RWPE-1 cells by exposure to N-methyl-N-nitrosourea (MNU): WPE1-NA22 (ATCC CRL-2849), WPE1-NB14 (ATCC CRL-2850), WPE1-NB11 (ATCC CRL-2851) and WPE1-NB26 (ATCC CRL-2852).The depositor reports that this cell line was screened for Hepatitis B and C, and human immunodeficiency viruses, and was found to be negative. |
| Clinical Data | male Caucasian, White 54 years |
| Antigen Expression | kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen) (upon exposure to androgen) |
| Receptor Expression | androgen receptor, expressed (upregulated upon exposure to androgen) |
| Genes Expressed | WPE1-NB26 cells were derived from RWPE-1 cells (ATCC CRL-11609) after exposure to N-methyl-N-nitrosourea (MNU) [PubMed: 11304724]. cytokeratin 18,cytokeratin 8,kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen),The RWPE2-W99 cell line was derived, in 1999, from the RWPE-2 (ATCC CRL-11610) cell line by cloning in soft agar to select cells that show high expression of Ki-ras. |
| Cellular Products | cytokeratin 18 cytokeratin 8 |
| Tumorigenic | Yes |
| Effects | Yes, in nude mice (RWPE2-W99 cells form small tumors when injected subcutaneously, with Matrigel, in nude mice.)(Mukta M Webber, personal communication) Yes, the cells form colonies in soft agar (small colonies are formed)(Mukta M Webber, personal communication) |
| Comments | The RWPE2-W99 cell line was derived, in 1999, from the RWPE-2 (ATCC CRL-11610) cell line by cloning in soft agar to select cells that show high expression of Ki-ras. RWPE2-W99 cells show strong expression of Ki-ras and have characteristics similar to those of RWPE-2 cells.
RWPE-2 cells were derived from RWPE-1 cells (ATCC CRL-11609) by transformation with Ki-ras using the Kirsten murine sarcoma virus (Ki-MuSV) to establish the RWPE-2 cells (PubMed: 9214605). Epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome to establish the RWPE-1 cell line (ATCC CRL-11609). A family of tumorigenic cell lines that mimics multiple steps in prostate cancer progression was also derived from RWPE-1 cells by exposure to N-methyl-N-nitrosourea (MNU): WPE1-NA22 (ATCC CRL-2849), WPE1-NB14 (ATCC CRL-2850), WPE1-NB11 (ATCC CRL-2851) and WPE1-NB26 (ATCC CRL-2852). The depositor reports that this cell line was screened for Hepatitis B and C, and human immunodeficiency viruses, and was found to be negative. |
| Complete Growth Medium | The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF).
To make the complete growth medium, you will need to add the following components to the base medium:
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| Subculturing | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 48 hours
Note: Subculture cells before they reach confluence. Do not allow cells to become confluent. |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 15% fetal bovine serum and 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 13 D13S317: 8,14 D16S539: 9,11 D5S818: 12,15 D7S820: 10,11 THO1: 8,9.3 TPOX: 8,11 vWA: 14,18 |
| Population Doubling Time | 36 hours |
| Name of Depositor | MM Webber |
| Year of Origin | 1999 |
| References | Bello D, et al. Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis 18: 1215-1223, 1997. PubMed: 9214605 Webber MM, et al. Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. Carcinogenesis 18: 1225-1231, 1997. PubMed: 9214606 Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420 Okamoto M, et al. Interleukin-6 and epidermal growth factor promote anchorage-independent growth of immortalized human prostatic epithelial cells treated with N-methyl-N-nitrosourea. Prostate 35: 255-262, 1998. PubMed: 9609548 Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29: 386-394, 1996. PubMed: 8977636 Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed: 9018337 Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152 Kremer R, et al. ras Activation of human prostate epithelial cells induces overexpression of parathyroid hormone-related peptide. Clin. Cancer Res. 3: 855-859, 1997. PubMed: 9815759 Jacob K, et al. Osteonectin promotes prostate cancer cell migration and invasion: a possible mechanism for metastasis to bone. Cancer Res. 59: 4453-4457, 1999. PubMed: 10485497 Achanzar WE, et al. Cadmium induces c-myc, p53, and c-jun expression in normal human prostate epithelial cells as a prelude to apoptosis. Toxicol. Appl. Pharmacol. 164: 291-300, 2000. PubMed: 10799339 Achanzar WE, et al. Cadmium-induced malignant transformation of human prostate epithelial cells. Cancer Res. 61: 455-458, 2001. PubMed: 11212230 Bello-DeOcampo D, et al. Laminin-1 and alpha6beta1 integrin regulate acinar morphogenesis of normal and malignant human prostate epithelial cells. Prostate 46: 142-153, 2001. PubMed: 11170142 Webber MM, et al. Human cell lines as an in vitro/in vivo model for prostate carcinogenesis and progression. Prostate 47: 1-13, 2001. PubMed: 11304724 upon exposure to androgen Quader ST, et al. Evaluation of the chemopreventive potential of retinoids using a novel in vitro human prostate carcinogenesis model. Mutat. Res. 496: 153-161, 2001. PubMed: 11551491 Webber MM, et al. A human prostatic stromal myofibroblast cell line WPMY-1: a model for stromal-epithelial interactions in prostatic neoplasia. Carcinogenesis 20: 1185-1192, 1999. PubMed: 10383888 Bello-DeOcampo D, et al. The role of alpha 6 beta 1 integrin and EGF in normal and malignant acinar morphogenesis of human prostatic epithelial cells. Mutat. Res. 480-481: 209-217, 2001. PubMed: 11506815 upregulated upon exposure to androgen Webber MM, et al. Modulation of the malignant phenotype of human prostate cancer cells by N-(4-hydroxyphenyl)retinamide (4-HPR). Clin. Exp. Metastasis 17: 255-263, 1999. PubMed: 10432011 Sharp RM, et al. N-(4-hydroxyphenyl)retinamide (4-HPR) decreases neoplastic properties of human prostate cells: an agent for prevention. Mutat. Res. 496: 163-170, 2001. PubMed: 11551492 Carruba G, et al. Regulation of cell-to-cell communication in non-tumorigenic and malignant human prostate epithelial cells. Prostate 50: 73-82, 2002. PubMed: 11816015 Achanzar WE, et al. Altered apoptotic gene expression and acquired apoptotic resistance in cadmium-transformed human prostate epithelial cells. Prostate 52: 236-244, 2002. PubMed: 12111698 Carruba G, et al. Intercellular communication and human prostate carcinogenesis. Ann. N.Y. Acad. Sci. 963: 156-168, 2002. PubMed: 12095941 Saladino F, et al. Connexin expression in nonneoplastic human prostate epithelial cells. Ann. N.Y. Acad. Sci. 963: 213-217, 2002. PubMed: 12095946 Hegarty PK, et al. Effects of cyclic stretch on prostatic cells in culture. J. Urol. 168: 2291-2295, 2002. PubMed: 12394777 Lugassy C, et al. Human melanoma cell migration along capillary-like structures in vitro: a new dynamic model for studying extravascular migratory metastasis. J. Invest. Dermatol. 119: 703-704, 2002. PubMed: 12230517 Brambila EM, et al. Chronic arsenic-exposed human prostate epithelial cells exhibit stable arsenic tolerance: mechanistic implications of altered cellular glutathione and glutathione S-transferase. Toxicol. Appl. Pharmacol. 183: 99-107, 2002. PubMed: 12387749 Achanzar WE, et al. Inorganic arsenite-induced malignant transformation of human prostate epithelial cells. J. Natl. Cancer Inst. 94: 1888-1891, 2002. PubMed: 12488483 RWPE2-W99 cells form small tumors when injected subcutaneously, with Matrigel, in nude mice. small colonies are formed Mukta M Webber, personal communication |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
