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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 5.4109997749328610

DESCRIPTION OF VECTOR:
Intact vector size: 5.411
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS416
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1323
terminator: CYC1, ->, 2005-2265
MCS: SalI...XbaI, ->, 2271-2328
promoter for expression: CYC1, <-, 2329-2616
marker(s): ampR, <-, 3863-4793
replicon: CEN6/ARSH4, ->, 4887-5391

Vector: p416 CYC1 (plasmid)

Promoters: Promoter for expression CYC1

Construction: pRS416

Marker(s):URA3,ampR

Construct size (kb): 5.410999774932861

Features: marker(s): URA3
marker(s): ampR
promoter for expression: CYC1
replicon: CEN6/ARSH4
MCS: SalI...XbaI
terminator: CYC1

YX-type (expression) shuttle vector

vector with low copy number

Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.1, 0.3; KpnI--5.4; EcoRI--5.4.

Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.

Low copy number shuttle expression vector.

Temperature: 37.0°C

component of:ATCC 87669