Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HindIII--4.4; BglII--4.4; ClaI--uncut.
Developed to simplify construction of hybrid CAT genes.
The ClaI site appears to have been destroyed during cloning.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Luckow B, Schutz G. CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements. Nucleic Acids Res. 15: 5490, 1987. PubMed: 3037497
