| Vector |
Construct size (kb): 5.400000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.4; PvuII--2.45, 0.9, 0.75, 0.4, 0.4, 0.45; HindIII--3.0, 2.35; BglI--1.5, 1.5, 1.25, 1.1; PstI--4.2, 1.1. Insert contains the following restriction sites (approximate kb from the 5' end): BglI--1.10; HindIII--0.05; NcoI--1.12; PstI--0.12, 1.17, 1.20; PvuII--0.42, 0.84, 1.24, 1.27, 2.18; SacI--1.75, 2.23; SmaI--1.15; SalI--0.26. Corresponds to nt 151-2543 of the published sequence (E2 splicing variant). Detects transcripts in human heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. |
