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Distribution host: Escherichia coli MC1066

Size (kb): 10.3179998397827200

DESCRIPTION OF VECTOR:
Intact vector size: 10.318
Type of vector: plasmid
Cloning sites: SacI SmaI SalI
Polylinker sites: EcoRI SacI AvaI KpnI SmaI BamHI XbaI SalI PstI HindIII
Other unique sites: NotI
Host range: Schizosaccharomyces pombe; Saccharomyces cerevisiae;
Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7, ->, 9016-9035
MCS: EcoRI...HindIII, ->, 10269-2
marker(s): GART, <-, 2-2091
replicon: 2 micron/LEU2-d, 2086-4780
marker(s): LEU2-d, ->, 3443-4537
marker(s): URA3, <-, 4860-5662
promoter for in vitro transcription: T3, <-, 5895-5914
promoter: lac, <-, 5984-6012
replicon: pMB1, 6338-6338
marker(s): ampR, <-, 7096-7956

Vector: YEpFAT7 (plasmid)

Promoters: Promoter for in vitro transcription T7

Marker(s):URA3,ampR,LEU2-d

Construct size (kb): 10.31799983978272

Features: marker(s): GART
marker(s): LEU2-d
marker(s): URA3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron/LEU2-d
replicon: pMB1
MCS: EcoRI...HindIII

Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.

Restriction digests of the clone give the following sizes (kb): BamHI--9.6, 1.0; SmaI--11.0; HindIII--6.2, 4.8.

Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.

One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.

Temperature: 37.0°C