| 產品名稱 |
pCK92 [hMCT1] |
| 商品貨號 |
B189485 |
| Designations |
pCK92 [hMCT1] |
| GenBank Number |
L31801
|
| Species |
Homo sapiens, human |
| Depositors |
JL Goldstein |
| Vector |
Construct size (kb): 5.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: SalI
Vector end: PstI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SLC16A1
Gene name: solute carrier family 16 (monocarboxylate transporters),
member 1
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 p13.2-p12
Tissue: heart
Type of DNA: cDNA
Insert 5' end: SalI
Insert 3' end: PstI
Insert size (kb): 2.5
Cross references: DNA Seq. Acc.: L31801 Insert lengths(kb): 2.5 Tissue: heart Gene product: mevalonate transporter(monocarboxylate transporter 1) [SLC16A1] |
| Insert Size (kb) |
2.5 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRV--4.1, 1.4; PstI--5.5; PstI/SalI--2.9, 2.6; XhoI--5.5; BamHI--5.5. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--0.2, 1.6. Contains the complete coding sequence plus the 3' untranslated region of approximately 1 kb. |
| References |
Garcia CK, et al. cDNA cloning of the human monocarboxylate transporter 1 and chromosomal localization of the SLC16A1 locus to 1p13.2-p12. Genomics 23: 500-503, 1994. PubMed: 7835905
Joseph L Goldstein, personal communication
|
