| 產品名稱 |
pLAFR1 cosmid in E. coli |
| 商品貨號 |
B189344 |
| Designations |
pLAFR1 cosmid in E. coli |
| Depositors |
FM Ausubel |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 21.60 Vector: pLAFR1 (cosmid) Construction: pRK270, pHC79 Marker(s):tetR Construct size (kb): 21.60 Features: marker(s): tetR
replicon: RK2, pMB1 |
| Applications |
shuttle vector vector for constructing genomic libraries |
| Comments |
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + tet plates at 30C and picking small colonies for liquid culture. A broad host range cosmid vector containing a relaxation complex site. |
| Media |
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
|
| Growth Conditions |
Temperature: 30.0°C |
| References |
Friedman AM, et al. Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene 18: 289-296, 1982. PubMed: 6290332
|
| Shipped |
freeze-dried |
| Shipping Information |
Shipped: ?Freeze dried E. coli containing the cosmid |
