| Insert |
DNA: genomic Insert lengths(kb): 40.0 Gene product: DNA Segment, single copy [D17S20] Alleles: B1, B2, A1, C1, C3, D1, D2, C2, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--19.4, 8.8 doublet, 6.5, 2.7, 0.8; EcoRI--15, 8.8, 7.5, 4.3, 3.4, 2.5, 2.1, 1.9, 1.4, 1.2, 1.1, 1, 0.8; SalI--greater than 25; BglI--11, 7.5, 3.8, 2.1, 1.7, 1.3, 0.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Probe used was a 7.6 kb TaqI fragment. Probe must be prehybridized with excess human DNA to compete out repeated sequences. Loci A, C, and D are in strong linkage disequilibrium. 29/40 (72%) individuals show heterozygosity at 1 or more loci. EcoRI detects an insertion-deletion polymorphism also detected by BamHI and BstEII. |
| References |
Litt M, White RL. A highly polymorphic locus in human DNA revealed by cosmid-derived probes. Proc. Natl. Acad. Sci. USA 82: 6206-6210, 1985. PubMed: 2994065
Buroker NE, et al. Cosmid 1-26 defines four RFLP's on chromosome 17q23-qter [HGM9 No. D17S20]. Nucleic Acids Res. 15: 9097, 1987. PubMed: 2891105
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