Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 Kit
Storage Conditions
-20°C (or -70°C for long-term storage)
Comments
The kit contains components that when added to Prostate Epithelial Cell Basal Medium (ATCC PCS-440-030) create a complete ATCC® Primary Cell Solutions™ culture environment for epithelial cells derived from normal human prostate. The serum-free medium formulation is designed to support normal prostate epithelial cell morphology as well as promote rapid growth and proliferation. No feeder layers, extracellular matrix proteins or other substrates are required.
The final concentration for each component in complete prostate epithelial cell growth medium is as follows:
L-Glutamine: 6 mM
Extract P: 0.4%
Epinephrine: 1.0 µM
rh TGF-α: 0.5 ng/mL
Hydrocortisone: 100 ng/mL
rh Insulin: 5 µg/mL
Apo-transferrin: 5 µg/mL
Complete Growth Medium
Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
Thaw the components of the growth kit just prior to adding to the basal medium. Warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates prior to adding to the basal medium.
Obtain one bottle of Prostate Epithelial Cell Basal Medium (485 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying with 70% ethanol.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component to the bottle of basal medium using a separate sterile pipette for each.
