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Cryptococcus amylolentus (van der Walt et al.) Golubev, anamorph

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產(chǎn)品名稱	Cryptococcus amylolentus (van der Walt et al.) Golubev, anamorph
商品貨號(hào)	B188753
Deposited As	Cryptococcus amylolentus (van der Walt et al.) Golubev
Strain Designations	NRRL Y-7784 [CBS 6039, IFO 10423, JCM 1690]
Application	Multigene phylogeny and phenotypic characterization.
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	freeze-dried
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Type Strain	yes
Preceptrol®	no
Genome Sequenced Strain	Yes
Comments	utilizes D-xylose, xylitol and D-xylonate Multigene phylogeny and phenotypic characterization. Genome sequencing strain (Broad Institute, USA)
Morphology	After 3 days on YM at 25°C, two colony types present on plates. One type off-white, smooth, mucoid, glistening; yeast cells subglobose or ovoid, single or in pairs, no pseudohyphae present. Second type white, raised, rough, dull; yeast cells subglobose or ovoid, single or in pairs, pseudohyphae present.
Medium	ATCC^® Medium 28: Emmons' modification of Sabouraud's agar ATCC^® Medium 200: YM agar or YM broth ATCC^® Medium 324: Malt extract agar
Growth Conditions	Temperature: 24°C to 26°C Atmosphere: Typical aerobic
Sequenced Data	18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence AAGGATCAGTAGAGAATAACGGACTTCGGTCCATATATCTACCCATCTACACCTGTGAACCGTATTTGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAAACTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAACATCTCAATCCTTCGGGTTTATGACCTGTTGGCATTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGACGTAATAAGTTTCGTTGGGCCTATGGGGTAGTTGTCGGCTTGCTTATAACAACCATCTTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAG D1D2 region of the 28S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTTCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACTGTGTCTAAGTTCCTTGGAATAGGATATCAAAGAGGGTGACAATCCCGTACTTGACACAATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTGCTTGGGAATGCAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAACCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCACGAGGAATGTAGCAATCTTCGGGTTGTGTTATAGCCTTGTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC
Name of Depositor	NRRL
Isolation	Frass of the tunnels of Sinoxylon ruficorne infesting Dichrostachys cinerea near Groblersdal, Transvaal, South Africa
Cross References	Nucleotide (GenBank) : AF105391 26S ribosomal RNA gene, partial sequence Nucleotide (GenBank) : AWGJ00000000 Cryptococcus amylolentus CBS 6039, whole genome shotgun sequencing project
References	van der Walt JP, et al. Six new Candida species from South African insect sources. Mycopathol. Mycol. Appl. 47: 221-236, 1972. van der Walt JP, Steyn RL. Note on D-xylonate utilization by ascomycetous and basidiomycetous yeasts. Syst. Appl. Microbiol. 13: 192-193, 1990. Fell JW, et al. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evol. Microbiol. 50: 1351-1371, 2000. PubMed: 10843082 Findley K, et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the Tremellales. Eukaryot Cell 8(3): 353-361, 2009. PubMed: 19151324 Guerreiro MA, et al. Molecular and genetic evidence for a tetrapolar mating system in the basidiomycetous yeast Kwoniella mangrovensis and two novel sibling species. Eukaryot Cell 12: 746-760, 2013. PubMed: 23524993 Findley K, et al. Discovery of a modified tetrapolar sexual cycle in Cryptococcus amylolentus and the evolution of MAT in the Cryptococcus species complex. PLoS Genet 8: e1002528, 2012. Scorzetti G, et al. Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res. 2: 495–517, 2002. PubMed: 12702266 Bovers M, et al. Six monophyletic lineages identified within Cryptococcus neoformans and Cryptococcus gattii by multi-locus sequence typing. Fungal Genet Biol 45: 400-421, 2008. PubMed: 18261945

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