For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). The insert contains nt 4190-4945 of Genbank J04423. The insert contains the following restriction sites (approximate kb from the 5' end): RsaI--0.14; SphI--0.48; SspI--0.65. The following enzymes are recommend for linearizing the plasmid for in vitro transcription: T3 = SmaI; T7 = XhoI. Restriction digests of the clone give the following sizes (kb): SmaI--3.4; SmaI/XhoI--2.7, 0.78; XhoI--3.4. |
