| 產品名稱 |
pCM185 |
| 商品貨號 |
B188093 |
| Designations |
pCM185 |
| Depositors |
E Herrero |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 7.7300000190734860 Vector: pCM185 (plasmid) Promoters: Promoter CMV Marker(s):TRP1,ampR Construct size (kb): 7.730000019073486 Features: marker(s): TRP1
marker(s): ampR
operator: tetO - seven repeats
other: tTA transcriptional activator
promoter: CMV
promoter: CYC1
replicon: ARS1
replicon: pMB1
MCS: BamHI...PstI
terminator: ADH1
terminator: CYC1
centromere: CEN4 |
| Applications |
YC-type (centromeric) shuttle vector expression vector |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--7.8; PstI--7.8; BamHI/XhoI--6.9, 0.8. Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Gari E, et al. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848, 1997. PubMed: 9234672
|
| Related Products |
component of:ATCC 87663 |
| Shipped |
frozen |
| Shipping Information |
?Frozen glycerol stock of E. coli containing the plasmid. |
