Restriction digests of the clone give the following sizes (kb): EcoRI--7.5; BamHI--7.5; HinddIII--5.4, 2.2. Cloned inserts can be transcribed from the S. cerevisiae RNase P RNA gene (RPR1) intragenic promoter to produce RPR1 leaders fused to RNA corresponding to inserted sequences. One of a series of shuttle vectors (ATCC 87167 - 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number. Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS424. |
