Restriction digests of the clone give the following sizes (kb): BamHI--14.0; EcoRI--14.0; HindIII--8.0, 4.3, 1.0, 0.9. The gene of interest can be inserted into pPbac by removal of the stuffer fragment which has a 5' SmaI site and a 3' BamHI site. pPbac is a baculovirus transfer vector that facilitates secretion of recombinant proteins in insect cell systems based on the presence of the human placental alkaline phosphatase leader signal. |
