| 產品名稱 |
HFBCO78 |
| 商品貨號 |
B186756 |
| Designations |
HFBCO78 |
| GenBank Number |
M85854
S76965
T05018
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 4.559999942779541 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: protein kinase inhibitor
Contains complete coding sequence?: Y
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.6
Cross references: DNA Seq. Acc.: M85854; EST: EST02378; Primer: M13 Forward;
THC id: THC169445
DNA Seq. Acc.: S76965; EST: EST02906; Primer: M13 Reverse;
THC id: THC169445
Nucleotides 1-307 of the insert correspond to
nucleotides 2079-1773 of S76965 with 98% identity.
DNA Seq. Acc.: S76965; EST: EST02378; Primer: M13 Forward;
THC id: THC169445
Nucleotides 1-235, 223-267, 260-312 of the insert correspond to
nucleotides 505-739, 726-770, 762-824 of S76965.
DNA Seq. Acc.: T05018; EST: EST02906; Primer: M13 Reverse;
THC id: THC169445 Insert lengths(kb): 1.600000023841858 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: protein kinase inhibitor |
| Insert Size (kb) |
1.600 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.1, 0.55. EST02378 matches GenBank accession MUSPKI with 91.48% identity over the sequence range: 1-235. This sequence record is for mouse cAMP-dependent protein kinase inhibitor. |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434
J C Venter, personal communication
|
