| 產(chǎn)品名稱(chēng) | Primary Dermal Microvascular Endothelial Cells; Normal, Human, Neonatal (HDMVECn) | |||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 商品貨號(hào) | B186336 | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Organism | Homo sapiens, human | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Tissue | Skin | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Cell Type | Endothelial | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Morphology | Cobblestone appearance with large dark nuclei; during proliferation, cells are small and evenly sized, display a high mitotic index and show no presence of smooth muscle cells. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Growth Properties | Adherent | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Biosafety Level | 1 [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens. ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223. Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.] Human Material Precaution All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
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| Disease | Normal | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Age | Neonatal | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Gender | Batch-specific | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Ethnicity | Batch-specific | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Applications | Drug screening, angiogenesis, oncology, inflammation, intercellular communication |
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| Product Format | frozen 1 mL | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Storage Conditions | -130°C or below | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Comments | The HDMVECn cells are cryopreserved in the third passage to ensure the greatest purity of the cell population, coupled with high viability and plating efficiency.
A complete solution to propagate dermal microvascular cells in low serum (5% FBS) conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Microvascular Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract (BBE), is recommended if a more "authentic," less defined culture medium is desired. |
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| Complete Growth Medium |
Table 1. If using the Microvascular Endothelial Cell Growth Kit-BBE (ATCC® PCS-110-040), add the indicated volume for each component:
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