| 產(chǎn)品名稱 |
D15 |
| 商品貨號 |
B185784 |
| Designations |
D15 |
| GenBank Number |
L10820
|
| Species |
Homo sapiens, human |
| Depositors |
PM Murphy |
| Vector |
Construct size (kb): 4.349999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: XhoI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: FPR1
Gene name: formyl peptide receptor 1
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.4
Tissue: HL-60 cell line
Disease: promyelocytic leukemia
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI linker
Insert 3' end: Modification: XhoI linker
Insert size (kb): 1.35
Cross references: DNA Seq. Acc.: L10820
Nucleotides 1-1350 of the insert correspond to
nucleotides 525-552, 5370-6599 of L10820. Insert lengths(kb): 1.350000023841858 Tissue: HL-60 cell line promyelocytic leukemia Disease: promyelocytic leukemia Gene product: formyl peptide receptor 1 [FPR1] |
| Insert Size (kb) |
1.350 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; XhoI--4.3; EcoRI/XhoI--2.9, 1.4; BamHI--4.3; PstI--3.2, 1.1. The insert includes the following restriction sites (approximate kb from the 5' end): NcoI--0.25; PvuII--1.0; PstI--1.14; SacI--1.18. Lacks exon 2 in the 5' untranslated region that is present in some alternatively spliced transcripts. Begins within exon 1 and contains the complete exon 3. |
| References |
Murphy PM, et al. Sequence and organization of the human N-formyl peptide receptor-encoding gene. Gene 133: 285-290, 1993. PubMed: 8224916
Murphy PM, McDermott D. Functional expression of the human formyl peptide receptor in Xenopus oocytes requires a complementary human factor. J. Biol. Chem. 266: 12560-12567, 1991. PubMed: 1712023
Murphy PM, et al. A structural homologue of the N-formyl peptide receptor. Characterization and chromosome mapping of a peptide chemoattractant receptor family. J. Biol. Chem. 267: 7637-7643, 1992. PubMed: 1373134
|
