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Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Kishimoto TK, et al. Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro. Blood 78: 805-811, 1991. PubMed: 1713515

Abbassi O, et al. Canine neutrophil margination mediated by lectin adhesion molecule-1 in vitro. J. Immunol. 147: 2107-2115, 1991. PubMed: 1717549

Abbassi O, et al. E-selectin supports neutrophil rolling in vitro under conditions of flow. J. Clin. Invest. 92: 2719-2730, 1993. PubMed: 7504692

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.