| 產(chǎn)品名稱 |
BT |
| 商品貨號(hào) |
B164048 |
| Organism |
Bos taurus, cow |
| Tissue |
turbinate |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
newborn |
| Strain |
Holstein |
| Storage Conditions |
liquid nitrogen vapor phase |
| Comments |
This line has been tested, and found negative for, Bovine viral diarrhea virus (BVDV). The cells should not be grown in medium containing serum of bovine origin. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
AW McClurkin |
| Deposited As |
Bos taurus |
| References |
McClurkin AW, et al. Comparison of low- and high-passage bovine turbinate cells for assay of bovine viral diarrhea virus. Arch. Virusforsch. 45: 285-289, 1974. PubMed: 4472511
Bermudez-Cruz R, et al. Characterization of an abundant, unique 1.7-kilobase bovine herpesvirus 4 (BHV-4) late RNA and mapping of a BHV-4 IE2 transactivator-binding site in its promoter-regulatory region. J. Virol. 71: 527-538, 1997. PubMed: 8985381
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