| 產品名稱 |
2H6-C2 |
| 商品貨號 |
B163689 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for the gD glycoprotein of BHV-1 and is used by the National Veterinary Services Laboratories of the USDA for in vitro potency testing of adjuvantated, inactivated BHV-1 vaccines. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Bovine herpesvirus 1 (BHV-1, also known as infectious bovine rhinotracheitis virus (IBRV)) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Bovine herpesvirus 1 (BHV-1, also known as infectious bovine rhinotracheitis virus (IBRV)) |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with BHV-1. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for the gD glycoprotein of BHV-1 and is used by the National Veterinary Services Laboratories of the USDA for in vitro potency testing of adjuvantated, inactivated BHV-1 vaccines. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2b |
| Name of Depositor |
CA Whetstone |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
. . Conf. Res. Workers Anim. Dis. : 54, 1987.
|
