| 產品名稱 |
16-10A1 |
| 商品貨號 |
B163589 |
| Organism |
hamster (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with and blocks the costimulatory activity of CD80. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against murine CD80 (B7-1) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against murine CD80 (B7-1) |
| Comments |
Animals were immunized with CD80 transfected CHO cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with and blocks the costimulatory activity of CD80. It does not affect the activity of CD86 (B7-2). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG |
| Name of Depositor |
H Reiser, BW Janicki |
| Deposited As |
hamster (B cell); mouse (myeloma) |
| References |
Razi-Wolf Z, et al. Expression and function of the costimulatory molecule B7 on murine Langerhans cells: evidence for an alternative CTLA-4 ligand. Eur. J. Immunol. 24: 805-811, 1994. PubMed: 7512030
Razi-Wolf Z, et al. Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells. Proc. Natl. Acad. Sci. USA 89: 4210-4214, 1992. PubMed: 1373896
Razi-Wolf Z, et al. Evidence for an additional ligand, distinct from B7, for the CTLA-4 receptor. Proc. Natl. Acad. Sci. USA 90: 11182-11186, 1993. PubMed: 7504299
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