Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
derived from wild type virus isolated from a virus-killed population of Lymantria dispar (gypsy moth), Abington, MA
Product Format
freeze-dried
Storage Conditions
-20°C or colder
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Comments
To propagate, rehydrate in water (or thaw), and feed to gypsy moth larvae (and collect hemolymph), or dissolve in 0.1 M Na2CO3, 0.2 M NaCl (pH 10.8) and inoculate gypsy moth cell culture (and collect cell lysate and supernate).
Effect on Host
Yes, in vivo effects: Kills 4th-5th instar Lymantria dispar larvae, 5-7 days
Yes, in vitro effects: nuclear swelling and inclusion bodies in cell culture, 3-4 days
Recommended Host
gypsy moth larvae
gypsy moth larvae and closely related lymantrids; IPLB LDFB, IPLB 652Y, and IPLB EIT cell cultures
