Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
?-70°C or colder
Comments
New Taxon.
It is recommended to determine viability by a PCR-based assay as agent does not produce cytopathic effects.
Effect on Host
No CPE produced
Recommended Host
Vero cells (ATCC CCL-81)
Growth Conditions
Temperature: 35°C
Recommendations for Infection: Plant cells 24 hours in advance and infect when cultures are 90-100% confluent. Remove medium and inoculate with an appropriate volume of Rickettsiae (e.g. 1 mL per 25 cm2) diluted in agent growth medium to provide an appropriate MOI (e.g. MOI of 50 for 50 Rickettsiae/ cell). Adsorb 1 hour at 800 x g at 20°C in a low speed centrifuge in closed buckets. End adsorption by adding agent growth medium to culture(s).
Incubation: Incubate infected culture(s) for 7-14 days at 35°C in a humidified 5% CO2 atmosphere.
Name of Depositor
L Izzard
Source
Tissue from the bat tick Argas dewae (soft tick), Southern Victoria, Australia.
