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8B1B.1

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編號:B162400

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產品名稱	8B1B.1
商品貨號	B162400
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	The antibody is specific for the structural protein VP7.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus
Cellular Products	immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus
Tumorigenic	Yes
Effects	Yes, in BALB/c mice
Comments	Animals were immunized with bluetongue virus (serotype 17). Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody is specific for the structural protein VP7.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 6 x 10⁴ viable cells/mL. Maintain cultures at a cell concentration between 5 x 10⁴and 5 x 10⁶cells/mL. Medium Renewal: Two to three times weekly
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	IgG2b
Name of Depositor	JA House
Deposited As	mouse (B cell); mouse (myeloma)
References	Appleton JA, Letchworth GJ. Monoclonal antibody analysis of serotype-restricted and unrestricted bluetongue viral antigenic determinants. Virology 124: 286-299, 1983. PubMed: 6186076 House C, et al. Detection of bluetongue group-specific antibody by competitive ELISA. J. Vet. Diagn. Invest. 2: 137-139, 1990. PubMed: 1965579 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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