Restriction digests of the clone give the following sizes (kb): EcoRI--4.9, 0.9; KpnI--6.0; PstI--4.8, 1.2.
Plasmid vector allowing for direct selection for hygromycin B resistance after transfection of eukaryotic cell lines.
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References
Blochlinger K, Diggelmann H. Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells. Mol. Cell. Biol. 4: 2929-2931, 1984. PubMed: 6098829
