| 產(chǎn)品名稱 |
Influenza B virus |
| 商品貨號(hào) |
B162182 |
| Classification |
Orthomyxoviridae, Influenzavirus B |
| Agent |
Influenza B virus |
| Strain |
B/Mass/3/66 |
| Applications |
Influenza Research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Please see ATCC Virology Guide for more detailed information on propagation |
| Effect on Host |
Hemagglutinates chicken red blood cells and possible death of chicken embryo |
| Recommended Host |
10 day old incubated specific pathogen free embryonated chicken eggs |
| Growth Conditions |
Temperature: 33°C
Recommendations for Infection: Dilute virus with PBS prior to inoculation. While candling the eggs, draw a pencil line around the air sac. Using an 18.5 gauge needle, poke a hole into the air sac. Using a 1.0 mL syringe fitted with a 22.5 gauge needle, insert the needle through the hole and into the allantoic cavity. Inject 0.2 mL of diluted inoculum into the allantoic cavity. Seal the hole with nail polish or sterile tape. Incubate eggs at 33°C 2 days, checking embryo viability every 12-18 hours and discard non-viable eggs. To harvest allantoic fluid, refrigerate eggs for at least 2 hours post incubation. Use sterile scissors to cut away the shell around the air sack. Aspirate the allantoic fluid with a syringe or a pipette. Avoid capturing blood or yolk in the allantoic fluid.
Incubation: 2 days with humidity |
| Name of Depositor |
R.I.T. - Recherche et Industrie Therapeutiques |
| Source |
Throat washings from patient in US, 1966 |
| Year of Origin |
1966 |
| References |
. . NCDC Influenza-Respiratory Disease Surveillance Report 83: 4, 1967.
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