| 產品名稱 |
pGC24 plasmid in E. coli |
| 商品貨號 |
B161729 |
| Designations |
pGC24 plasmid in E. coli |
| Species |
Aspergillus awamori Nakazawa |
| Depositors |
Cetus Corp., A Belt, Cetus Corp. |
| Applications |
produces protein glucoamylase |
| Vector |
Construct size (kb): 6.55 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTRP3
Intact vector size: 4.460
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: HindIII
Polylinker sites:
Other unique sites: BamHI
Construction: pVH153, pBR322
Host range: Escherichia coli
Features (with orientation and position when available):
promoter: trp, ->
ribosome-binding site: , ->
restriction site: HindIII, ->
marker(s): tetR, ->
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Aspergillus awamori
Gene name: glucoamylase
Contains complete coding sequence?: U
Strain: NRRL 15271
Type of DNA: cDNA
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 2.11
Cross references: Insert lengths(kb): 2.10 Gene product: glucoamylase Target Gene: glucoamylase |
| Insert Size (kb) |
2.110 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Insert contains internal PstI and BamHI sites. Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 2.4; PstI--5.2, 1.9; BamHI--5.8, 1.1. Expresses the gene product under the control of the Escherichia coli trp promoter. The vector was constructed by inserting an EcoRI/ClaI fragment with Escherichia coli trp promoter and RBS into pBR322. A HindIII fragment of about 2.2 kb containing the gene with poly(A) signal was cloned into the HindIII site. |
| References |
Nunberg J, et al. Glucoamylase cDNA. US Patent 4,794,175 dated Dec 27 1988
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
